Seck, a summer arrival on a free transfer, slots in at left-back for the match at Bucharest's Arena Nationala, with Emerson Palmieri moving into midfield alongside fellow Brazilian Gerson and Kevin Strootman.
Francesco Totti leads the attack alongside Manuel Iturbe and Stephan El Shaarawy.
Roma have already secured top spot in Group E, and with it progression into the Europa League knockout stages.
See the full line-up in the graphic below.
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