The game, hosted at the Giulio Onesti sports centre, finished 1-0 to Betty Bavagnoli's side - thanks to Lindsey Thomas's goal early in the second half.
With the start of the season still three weeks away, the match was played over two halves of 35 minutes.
Manuela Giugliano, Andressa Alves and Elisa Bartoli were among a number of players unavailable for the match, having joined up with their respective national team sides.
The Giallorosse open the season on September 14 against AC Milan.
ROMA WOMEN XI to face Roma CF: Ceasar; Erzen, Corrado, Di Criscio, Cunsolo; Greggi, Bernauer, Hegerberg; Corelli, Thestrup, Thomas.
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