The two men, both 40, faced each other numerous times in the fixture during Nesta's early career at Lazio.
Now retired, Nesta admitted he is amazed that Totti is still playing at the highest level.
"Totti is the same age as me and he's still playing, while I'm coaching now," Nesta said. "He is special and blessed with extraordinary ability.
"At 40 years old, he's enjoying himself and spreading that enjoyment to others."
Nesta is looking forward to an entertaining game on Sunday.
"The Rome Derby is massive, just like the one in Milan," he added. "I played for ten years for both Lazio and AC Milan. The Biancocelesti are the club of my heart because I grew up there, but I won so much with Milan."
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