Luciano Spalletti has confirmed his group for the match - with Antonio Rudiger (suspended) the main absentee.
Alessandro Florenzi (injured) and Clement Grenier (ineligible) are the other senior players not included for the last-16 tie.
See the full squad list below.
AS Roma squad to face Olympique Lyon:
Alisson Becker
Bogdan Lobont
Wojciech Szczesny
Kostas Manolas
Emerson Palmieri
Mário Rui
Federico Fazio
Thomas Vermaelen
Bruno Peres
Juan Jesus
Gerson
Daniele De Rossi
Diego Perotti
Kevin Strootman
Leandro Paredes
Radja Nainggolan
Mohamed Salah
Stephan El Shaarawy
Francesco Totti
Edin Dzeko
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