The Giallorossi are looking to overturn a 2-1 deficit at the Stadio Olimpico, and reach the quarter-finals of the competition for the fourth time in their history.
Coach Eusebio Di Francesco has opted for Perotti in attack, with Edin Dzeko and Federico Fazio both returning to the starting line-up after being suspended for the Friday win over Torino.
Daniele De Rossi captains the side.
See the full line-up in the graphic below.
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