Eusebio Di Francesco's side, who finished second in Group G in the group stage of the competition, will face Porto in the last-16.
The first leg takes place in Rome on February 12, with the return leg in the Portuguese city taking place on March 6.
Here is the list of players registered:
1 OLSEN Robin Patrick
2 KARSDORP Rick
4 CRISTANTE Bryan
5 JESUS NUNES Juan Guilherme
7 PELLEGRINI Lorenzo
8 PEROTTI ALMEIRA Diego
9 DZEKO Edin
11 KOLAROV Aleksandar
14 SCHICK Patrik
15 MARCANO SIERRA Ivan
16 DE ROSSI Daniele
17 UNDER Cengiz
18 SANTON Davide
19 CORIC Ante
20 FAZIO Federico Julian
22 ZANIOLO Nicolò
24 FLORENZI Alessandro
27 PASTORE Javier Matias
34 KLUIVERT Justin Dean
42 NZONZI Steven Nkemboanza Mike Christopher
44 MANOLAS Konstantinos
63 FUZATO Daniel Cerantula
83 MIRANTE Antonio
92 EL SHAARAWY Stephan
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