The Giallorossi are heading to Manchester for Thursday's Europa League semi-final first leg, where they will face Manchester United.
The game at Old Trafford gets underway at 21:00 CEST.
Fans began arriving outside the club's training centre at Trigoria in the late morning, and numbered in the hundreds by the time Paulo Fonseca's side were preparing to depart.
A section of supporters unfurled a banner, which read:
Anche se non sentirete la nostra voce... guardate la Lupa che avete sul petto... Roma è con voi!
Even if you won't hear our voices, just look at the wolf on your chest - Rome is with you all!
As fans chanted and cheered outside the training complex, a number of players took to the balcony atop one of the buildings to salute the fans.
When the team bus left soon after, the send-off was electric.
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The team will arrive in Manchester later on Wednesday evening.
Fonseca and former Manchester United defender Chris Smalling will then hold a pre-match press conference at Old Trafford.
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