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Speaking ahead of Thursday's last-16 tie against Sampdoria, the Giallorossi's entrance in this year's competition, Spalletti rejected any suggestion that the game would be an opportunity to give some members of his squad more playing time.
"There are three trophies up for grabs in Italy. Tomorrow's contest is a knockout game," Spalletti said. "We can't allow ourselves to do any experiments when you don't know what the final result will be.
"The players not in the side are the same as those who are playing. I've had the fortune of lifting a few trophies in my time and when you raise one above your head, it seems as if there's a whole new life opening up in front of you. You feel more self-esteem, you feel stronger and more confident.
"Roma have to win, Roma need to win, not for the taste of victory in itself but for the people and their love and affection."
Spalletti was willing to admit that Alisson will start in goal at the Olimpico, while full-back Mario Rui is likely to make his club debut in some capacity, but spoke extensively about the motivation the club must have for the cup competition.
"This club has to capitalise on every moment in its existence," he added. "Roma shouldn't aim for the trophy cabinet, but rather work towards constant automatic movement from a good training session to a victory, thus building self-confidence to win the next match. A tackle that gives you strength to go and win the next one, a goal that turns into desire to get two. Perpetual motion.
"We have watermills in Tuscany; the more water you give them the more energy they produce. We need to do this. We're not in a situation where we can say that those who haven't played a lot need to feature tomorrow. The guys who will get the victory are those who will play tomorrow.
"We have to be fully professional in every second of our lives at Roma because that's what needs to be done. If the club wants to grow, it needs to do this. Recently we've been on the right track in this respect."
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