De Rossi will join Radja Nainggolan - who was omitted from the squad as punishment for his recent conduct - in missing the game at the Stadio Olimpico.
Gregoire Defrel remains absent as he continues his recovery from a knee injury.
The game on Saturday gets underway at 18:00 CET.
See the 21-man squad available for the game below.
Alisson Becker
Bogdan Lobont
Lukasz Skorupski
Juan Jesus
Aleksandar Kolarov
Hector Moreno
Federico Fazio
Bruno Peres
Emerson Palmieri
Kostas Manolas
Leandro Castan
Kevin Strootman
Lorenzo Pellegrini
Maxime Gonalons
Alessandro Florenzi
Gerson
Diego Perotti
Edin Dzeko
Patrik Schick
Cengiz Under
Stephan El Shaarawy
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