After a 4-0 win in the first leg a few weeks ago, the Giallorosse know a professional display should be enough to see them through.
Kick-off is at 14:30 CET.
"We know Florentia will come here to give it a go," said coach Betty Bavagnoli.
"In football there are many stories we could talk about where there have been comebacks in situations like this. So they will come here to play, to try, and we will have to make sure we are focused and prepared.
"We absolutely don't want to let this opportunity be squandered."
The game will be broadcast live on Roma TV+, and also on Twitter.
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