Summer signings Gianluca Mancini, Leonardo Spinazzola and Amadou Diawara are all included - while there are also starts for Mirko Antonucci and Gregoire Defrel.
Alessandro Florenzi captains the side.
The game, which gets underway at 20:00 CEST, is being broadcast live on the club's Twitter and Facebook profiles.
Roma XI to face Athletic Club: Mirante; Florenzi, Mancini, J. Jesus, Spinazzola; Pellegrini, Diawara; Cengiz, Antonucci, Perotti; Defrel.
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