Spalletti confirmed on Wednesday that the last-16 match, which gets underway at the Stadio Olimpico at 2100 CET, will see Alisson start in goal - while full-back Mario Rui is likely to make his debut for the club at some point in the evening.
See the full squad list below.
AS Roma squad to face Sampdoria:
Alisson Becker
Bogdan Lobont
Wojciech Szczesny
Emerson Palmieri
Mario Rui
Federico Fazio
Kostas Manolas
Moustapha Seck
Bruno Peres
Juan Jesus
Antonio Rudiger
Thomas Vermaelen
Daniele De Rossi
Gerson
Leandro Paredes
Kevin Strootman
Radja Nainggolan
Diego Perotti
Stephan El Shaarawy
Francesco Totti
Edin Dzeko
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