The France international, who was ultimately signed too late to be eligible for the midweek Coppa Italia win over Cesena, is in Luciano Spalletti's 20-man group for the game at the Stadio Olimpico.
Fellow midfielder Gerson, who like Grenier played for the Primavera at the weekend, is also included - but Thomas Vermaelen misses out with a hand injury.
The match against the Viola gets underway at 20:45 CET.
Update: Mohamed Salah returned to Rome early, and as a result has been added to the squad for the game.
See the full squad list below.
AS Roma squad to face Fiorentina:
Alisson Becker
Bogdan Lobont
Wojciech Szczesny
Emerson Palmieri
Mario Rui
Federico Fazio
Kostas Manolas
Bruno Peres
Juan Jesus
Antonio Rudiger
Daniele De Rossi
Gerson
Leandro Paredes
Kevin Strootman
Radja Nainggolan
Diego Perotti
Clement Grenier
Stephan El Shaarawy
Francesco Totti
Edin Dzeko
Mohamed Salah
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