Florenzi is named in the squad for the first time in 10 months, after suffering two serious knee injuries. On Friday Di Francesco indicated the Italy international is not ready to start the game, but could play a role at some point.
With both Bruno Peres and Nura Abdullahi ruled out through injury, defender Stefano Ciavattini has been added to the matchday party. He is joined by forward Mirko Antonucci, another member of Alberto De Rossi's Under-19 side.
Saturday's game gets underway at 20:45 CEST.
See the full 23-man squad below.
Alisson Becker
Bodgan Lobont
Lukasz Skorupski
Juan Jesus
Aleksandar Kolarov
Hector Moreno
Federico Fazio
Stefano Ciavattini
Kostas Manolas
Radja Nainggolan
Kevin Strootman
Lorenzo Pellegrini
Daniele De Rossi
Maxime Gonalons
Alessandro Florenzi
Gerson
Diego Perotti
Edin Dzeko
Cengiz Under
Gregoire Defrel
Marco Tumminello
Mirko Antonucci
Stephan El Shaarawy
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