De Rossi missed the weekend victory over Empoli with a back injury sustained while on international duty with Italy, but trained with the group on Monday and has been included in the squad for the decisive derby clash.
Kevin Strootman is the other addition from the weekend squad, after he missed the game due to suspension.
Tuesday's game gets underway at 20:45 CST.
AS Roma squad to face Lazio:
Alisson Becker
Bogdan Lobont
Wojciech Szczesny
Kostas Manolas
Emerson Palmieri
Mário Rui
Federico Fazio
Thomas Vermaelen
Bruno Peres
Juan Jesus
Antonio Rudiger
Gerson
Daniele De Rossi
Diego Perotti
Kevin Strootman
Clement Grenier
Leandro Paredes
Radja Nainggolan
Mohamed Salah
Stephan El Shaarawy
Francesco Totti
Edin Dzeko
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